Maps have been made showing the order of am mutant sites using (a) frequencies of am+ recombinants from crosses between am mutants and (b) the modes of distribution of the am+ recombinants among the two parental and two recombinant classes with respect to the flanking markers inos and sp.
It is possible to arrange the sites in an order such that, in almost all the crosses yielding useful numbers of am+ recombinants, the sp allele originally in coupling with the distal am mutant site occurs in the majority of the recombinants. No such consistent pattern was found with respect to the inos marker. The order obtained by reference to sp agreed with the best order deducible from recombination frequencies.
The data are consistent with the hypothesis that am+ recombinants arise by a process of gene conversion, that there is a gradient of conversion frequencies from the right (inos or distal) end of the gene to the left (sp or proximal) end, and that conversion tends to be associated (though less than 50% of the time) with crossing-over, especially on the distal side.
There is no obvious relationship between the map position of a given am mutant and the properties of the protein product of the mutant gene.